Concurrent amplification of cyclin E and CDK2 genes in colorectal carcinomas
The genetic changes of cyclin A, Dl, E and CDK2 were examined in human colorectal carcinomas by Southern‐blot analysis. Gene amplification of cyclin E was detected in 5 of 53 (9.4%) primary colorectal carcinoma tissues. Interestingly, in 3 of 5 tumors showing cyclin E gene amplification, the CDK2 ge...
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Veröffentlicht in: | International journal of cancer 1995-07, Vol.62 (1), p.25-28 |
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Sprache: | eng |
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Zusammenfassung: | The genetic changes of cyclin A, Dl, E and CDK2 were examined in human colorectal carcinomas by Southern‐blot analysis. Gene amplification of cyclin E was detected in 5 of 53 (9.4%) primary colorectal carcinoma tissues. Interestingly, in 3 of 5 tumors showing cyclin E gene amplification, the CDK2 gene was amplified simultaneously with rearrangements. No obvious correlation was detected between gene amplification and clinico‐pathological features of colorectal carcinomas. Out of 7 colon carcinoma cell lines, 2 showed gene amplification of cyclin E without gene amplification of CDK2. No amplification of cyclin A or Dl gene was found in any of the colorectal carcinoma tissues or colon carcinoma cell lines. Our results suggest that the concurrent amplification of cyclin E and CDK2 genes may play a role in colorectal carcinogenesis. © 1995 Wiley‐Liss Inc. |
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ISSN: | 0020-7136 1097-0215 |
DOI: | 10.1002/ijc.2910620107 |