Protein‐A antibody‐binding (PAAB) technique for detection of tumor‐associated membrane antigen (TAMA) of lewis lung carcinoma
Xenoantisera were prepared in rabbits against intact paraformaldehyde‐fixed Lewis lung carcinoma (LLCa) cells and membrane solubilized proteins extracted with Triton X‐100 and 3M KCI. The antisera were analyzed for antibodies to tumor‐associated membrane antigens (TAMA) by means of a solid‐phase rad...
Gespeichert in:
Veröffentlicht in: | International journal of cancer 1980-01, Vol.25 (1), p.141-145 |
---|---|
Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 145 |
---|---|
container_issue | 1 |
container_start_page | 141 |
container_title | International journal of cancer |
container_volume | 25 |
creator | Veltri, Robert W. McKolanis, John R. Rockoff, S. David McIntire, K. Robert |
description | Xenoantisera were prepared in rabbits against intact paraformaldehyde‐fixed Lewis lung carcinoma (LLCa) cells and membrane solubilized proteins extracted with Triton X‐100 and 3M KCI. The antisera were analyzed for antibodies to tumor‐associated membrane antigens (TAMA) by means of a solid‐phase radioimmunoassay (RIA). The immunoassay employed 125I‐labelled Staphylococcus aureus protein A (SPA) to detect specific antibody binding to glutaraldehyde‐fixed Lewis lung tumor target cells. The protein‐A antibody‐binding (PAAB) method is rapid, sensitive and reproducible. The PAAB revealed a possible LLCa‐TAMA as evidenced by the reactivity of xenoantisera raised to LL paraformaldehyde tumor cell vaccine (TCV) and LL tumor Triton X‐I00 (TTri) and 3M KCI (TKCI) extracts of membrane preparations. The reactivity of our antisera followed linear dose‐response kinetics but the degree of reactivity decreased from anti‐TCV to anti‐TTri to anti‐TKCI respectively. The LL‐TAMA demonstrated specificity since our antisera did not significantly cross‐react with normal C57BL/6 cellular antigens or other known murine tumor‐associated surface antigens of virally and chemically‐induced tumor systems. |
doi_str_mv | 10.1002/ijc.2910250119 |
format | Article |
fullrecord | <record><control><sourceid>wiley_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1002_ijc_2910250119</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>IJC2910250119</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2879-aba06b84a380ee312290c8eaebfd9fec073e8f4d083415addc7392a7d8959db03</originalsourceid><addsrcrecordid>eNqFkL1OwzAUhS0EEqWwMnssQ8q1kzTxGCp-ioroUObIsW-Kq8YGO1XVDYkX4Bl5ElKKBBvT0ZG-7wyHkHMGQwbAL81SDblgwFNgTByQHgORRcBZekh6HQBRxuLRMTkJYQkdkkLSI-8z71o09vPto6DStqZyetuVylht7IIOZkVxdUFbVM_WvK6R1s5TjV1vjbPU1bRdN853hgzBKSNb1LTBpvLS4vfgAi0dzIuH4mJHr3BjAl2tu2klvTLWNfKUHNVyFfDsJ_vk6eZ6Pr6Lpo-3k3ExjRTPMxHJSsKoyhMZ54AYM84FqBwlVrUWNSrIYszrREMeJyyVWqssFlxmOhep0BXEfTLc7yrvQvBYly_eNNJvSwbl7sKyu7D8vbATxF7YmBVu_6HLyf34j_sFWOJ5pA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Protein‐A antibody‐binding (PAAB) technique for detection of tumor‐associated membrane antigen (TAMA) of lewis lung carcinoma</title><source>Access via Wiley Online Library</source><creator>Veltri, Robert W. ; McKolanis, John R. ; Rockoff, S. David ; McIntire, K. Robert</creator><creatorcontrib>Veltri, Robert W. ; McKolanis, John R. ; Rockoff, S. David ; McIntire, K. Robert</creatorcontrib><description>Xenoantisera were prepared in rabbits against intact paraformaldehyde‐fixed Lewis lung carcinoma (LLCa) cells and membrane solubilized proteins extracted with Triton X‐100 and 3M KCI. The antisera were analyzed for antibodies to tumor‐associated membrane antigens (TAMA) by means of a solid‐phase radioimmunoassay (RIA). The immunoassay employed 125I‐labelled Staphylococcus aureus protein A (SPA) to detect specific antibody binding to glutaraldehyde‐fixed Lewis lung tumor target cells. The protein‐A antibody‐binding (PAAB) method is rapid, sensitive and reproducible. The PAAB revealed a possible LLCa‐TAMA as evidenced by the reactivity of xenoantisera raised to LL paraformaldehyde tumor cell vaccine (TCV) and LL tumor Triton X‐I00 (TTri) and 3M KCI (TKCI) extracts of membrane preparations. The reactivity of our antisera followed linear dose‐response kinetics but the degree of reactivity decreased from anti‐TCV to anti‐TTri to anti‐TKCI respectively. The LL‐TAMA demonstrated specificity since our antisera did not significantly cross‐react with normal C57BL/6 cellular antigens or other known murine tumor‐associated surface antigens of virally and chemically‐induced tumor systems.</description><identifier>ISSN: 0020-7136</identifier><identifier>EISSN: 1097-0215</identifier><identifier>DOI: 10.1002/ijc.2910250119</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><ispartof>International journal of cancer, 1980-01, Vol.25 (1), p.141-145</ispartof><rights>Copyright © 1980 Wiley‐Liss, Inc., A Wiley Company</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2879-aba06b84a380ee312290c8eaebfd9fec073e8f4d083415addc7392a7d8959db03</citedby><cites>FETCH-LOGICAL-c2879-aba06b84a380ee312290c8eaebfd9fec073e8f4d083415addc7392a7d8959db03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fijc.2910250119$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fijc.2910250119$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Veltri, Robert W.</creatorcontrib><creatorcontrib>McKolanis, John R.</creatorcontrib><creatorcontrib>Rockoff, S. David</creatorcontrib><creatorcontrib>McIntire, K. Robert</creatorcontrib><title>Protein‐A antibody‐binding (PAAB) technique for detection of tumor‐associated membrane antigen (TAMA) of lewis lung carcinoma</title><title>International journal of cancer</title><description>Xenoantisera were prepared in rabbits against intact paraformaldehyde‐fixed Lewis lung carcinoma (LLCa) cells and membrane solubilized proteins extracted with Triton X‐100 and 3M KCI. The antisera were analyzed for antibodies to tumor‐associated membrane antigens (TAMA) by means of a solid‐phase radioimmunoassay (RIA). The immunoassay employed 125I‐labelled Staphylococcus aureus protein A (SPA) to detect specific antibody binding to glutaraldehyde‐fixed Lewis lung tumor target cells. The protein‐A antibody‐binding (PAAB) method is rapid, sensitive and reproducible. The PAAB revealed a possible LLCa‐TAMA as evidenced by the reactivity of xenoantisera raised to LL paraformaldehyde tumor cell vaccine (TCV) and LL tumor Triton X‐I00 (TTri) and 3M KCI (TKCI) extracts of membrane preparations. The reactivity of our antisera followed linear dose‐response kinetics but the degree of reactivity decreased from anti‐TCV to anti‐TTri to anti‐TKCI respectively. The LL‐TAMA demonstrated specificity since our antisera did not significantly cross‐react with normal C57BL/6 cellular antigens or other known murine tumor‐associated surface antigens of virally and chemically‐induced tumor systems.</description><issn>0020-7136</issn><issn>1097-0215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><recordid>eNqFkL1OwzAUhS0EEqWwMnssQ8q1kzTxGCp-ioroUObIsW-Kq8YGO1XVDYkX4Bl5ElKKBBvT0ZG-7wyHkHMGQwbAL81SDblgwFNgTByQHgORRcBZekh6HQBRxuLRMTkJYQkdkkLSI-8z71o09vPto6DStqZyetuVylht7IIOZkVxdUFbVM_WvK6R1s5TjV1vjbPU1bRdN853hgzBKSNb1LTBpvLS4vfgAi0dzIuH4mJHr3BjAl2tu2klvTLWNfKUHNVyFfDsJ_vk6eZ6Pr6Lpo-3k3ExjRTPMxHJSsKoyhMZ54AYM84FqBwlVrUWNSrIYszrREMeJyyVWqssFlxmOhep0BXEfTLc7yrvQvBYly_eNNJvSwbl7sKyu7D8vbATxF7YmBVu_6HLyf34j_sFWOJ5pA</recordid><startdate>19800115</startdate><enddate>19800115</enddate><creator>Veltri, Robert W.</creator><creator>McKolanis, John R.</creator><creator>Rockoff, S. David</creator><creator>McIntire, K. Robert</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19800115</creationdate><title>Protein‐A antibody‐binding (PAAB) technique for detection of tumor‐associated membrane antigen (TAMA) of lewis lung carcinoma</title><author>Veltri, Robert W. ; McKolanis, John R. ; Rockoff, S. David ; McIntire, K. Robert</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2879-aba06b84a380ee312290c8eaebfd9fec073e8f4d083415addc7392a7d8959db03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Veltri, Robert W.</creatorcontrib><creatorcontrib>McKolanis, John R.</creatorcontrib><creatorcontrib>Rockoff, S. David</creatorcontrib><creatorcontrib>McIntire, K. Robert</creatorcontrib><collection>CrossRef</collection><jtitle>International journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Veltri, Robert W.</au><au>McKolanis, John R.</au><au>Rockoff, S. David</au><au>McIntire, K. Robert</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protein‐A antibody‐binding (PAAB) technique for detection of tumor‐associated membrane antigen (TAMA) of lewis lung carcinoma</atitle><jtitle>International journal of cancer</jtitle><date>1980-01-15</date><risdate>1980</risdate><volume>25</volume><issue>1</issue><spage>141</spage><epage>145</epage><pages>141-145</pages><issn>0020-7136</issn><eissn>1097-0215</eissn><abstract>Xenoantisera were prepared in rabbits against intact paraformaldehyde‐fixed Lewis lung carcinoma (LLCa) cells and membrane solubilized proteins extracted with Triton X‐100 and 3M KCI. The antisera were analyzed for antibodies to tumor‐associated membrane antigens (TAMA) by means of a solid‐phase radioimmunoassay (RIA). The immunoassay employed 125I‐labelled Staphylococcus aureus protein A (SPA) to detect specific antibody binding to glutaraldehyde‐fixed Lewis lung tumor target cells. The protein‐A antibody‐binding (PAAB) method is rapid, sensitive and reproducible. The PAAB revealed a possible LLCa‐TAMA as evidenced by the reactivity of xenoantisera raised to LL paraformaldehyde tumor cell vaccine (TCV) and LL tumor Triton X‐I00 (TTri) and 3M KCI (TKCI) extracts of membrane preparations. The reactivity of our antisera followed linear dose‐response kinetics but the degree of reactivity decreased from anti‐TCV to anti‐TTri to anti‐TKCI respectively. The LL‐TAMA demonstrated specificity since our antisera did not significantly cross‐react with normal C57BL/6 cellular antigens or other known murine tumor‐associated surface antigens of virally and chemically‐induced tumor systems.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><doi>10.1002/ijc.2910250119</doi><tpages>5</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0020-7136 |
ispartof | International journal of cancer, 1980-01, Vol.25 (1), p.141-145 |
issn | 0020-7136 1097-0215 |
language | eng |
recordid | cdi_crossref_primary_10_1002_ijc_2910250119 |
source | Access via Wiley Online Library |
title | Protein‐A antibody‐binding (PAAB) technique for detection of tumor‐associated membrane antigen (TAMA) of lewis lung carcinoma |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T07%3A48%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-wiley_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Protein%E2%80%90A%20antibody%E2%80%90binding%20(PAAB)%20technique%20for%20detection%20of%20tumor%E2%80%90associated%20membrane%20antigen%20(TAMA)%20of%20lewis%20lung%20carcinoma&rft.jtitle=International%20journal%20of%20cancer&rft.au=Veltri,%20Robert%20W.&rft.date=1980-01-15&rft.volume=25&rft.issue=1&rft.spage=141&rft.epage=145&rft.pages=141-145&rft.issn=0020-7136&rft.eissn=1097-0215&rft_id=info:doi/10.1002/ijc.2910250119&rft_dat=%3Cwiley_cross%3EIJC2910250119%3C/wiley_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |