Transformation of mouse cells after infection with ultraviolet irradiation‐inactivated herpes simplex virus type 2

A transformed mouse cell line (H238) was obtained following the infection of 238 mouse cells with ultraviolet (UV) irradiation‐inactivated herpes simplex virus type 2 (HSV‐2). The transformed cells produced tumors with a 100% incidence within 8 weeks in 6‐week‐old syngeneic BALB/c mice at an inoculu...

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Veröffentlicht in:	International journal of cancer 1975-10, Vol.16 (4), p.526-538
Hauptverfasser:	Boyd, Ann Lewis, Orme, Thomas W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Viral Antibody Specificity Antigens, Viral Cell Line Cell Transformation, Neoplastic DNA, Neoplasm - isolation & purification DNA, Viral - isolation & purification In Vitro Techniques Mice Mice, Inbred BALB C Neoplasm Transplantation Neoplasms, Experimental - immunology Neutralization Tests Radiation Effects Simplexvirus - immunology Simplexvirus - radiation effects Transplantation, Isogeneic Ultraviolet Rays
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Zusammenfassung:	A transformed mouse cell line (H238) was obtained following the infection of 238 mouse cells with ultraviolet (UV) irradiation‐inactivated herpes simplex virus type 2 (HSV‐2). The transformed cells produced tumors with a 100% incidence within 8 weeks in 6‐week‐old syngeneic BALB/c mice at an inoculum of 1 × 106. Indirect immunofluorescence (IF) tests revealed the presence of HSV antigens in the transformed cells. Antibodies to HSV‐2 were found in the sera of tumor‐bearing animals by neutralization and IF techniques. Neither HSV‐2 infectious virus nor viral antigens could be detected by the transfer of transformed‐cell DNA into permissive cells.
ISSN:	0020-7136 1097-0215
DOI:	10.1002/ijc.2910160403