Cyclophosphamide treatment induces rejection of established P815 mastocytoma by enhancing CD4 priming and intratumoral infiltration of P1E/H‐2K d ‐specific CD8 + T cells
There is increasing evidence that the effect of chemotherapy on tumor growth is not cell autonomous but relies on the immune system. The objective of this study was therefore to decipher the cellular and molecular mechanisms underlying the role of innate and adaptive immunity in chemotherapy‐induced...
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Veröffentlicht in: | International journal of cancer 2014-06, Vol.134 (12), p.2841-2852 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | There is increasing evidence that the effect of chemotherapy on tumor growth is not cell autonomous but relies on the immune system. The objective of this study was therefore to decipher the cellular and molecular mechanisms underlying the role of innate and adaptive immunity in chemotherapy‐induced tumor rejection. Treatment of DBA/2 mice bearing P815 mastocytoma with cyclophosphamide induced rejection and long‐term protection in a CD4‐ and CD8‐dependent manner. A population of inflammatory‐type dendritic cells was dramatically expanded in the lymph nodes of mice that rejected the tumor and correlated with CD4‐dependent infiltration, in tumor bed, of tumor‐specific CD8
+
T lymphocytes. Our data point to a major role of CD4
+
T cells in inducing chemokine expression in the tumor, provoking migration of tumor‐specific CXCR3
+
CD8
+
T lymphocytes. Importantly, the analysis of CD8
+
T cells specific to P1A/H‐2L
d
and P1E/H‐2K
d
revealed that cyclophosphamide altered the P815‐specific CD8 T repertoire by amplifying the response specific to the mutated P1E antigen.
What's new?
Although chemotherapy and immunotherapy have been long considered as independent treatments, there is increasing evidence that the success of chemotherapy depends, at least partially, on the immune system. This study investigated the role of immunity in chemotherapy‐induced tumor rejection. Cyclophosphamide was shown to induce rejection of P815 tumor in mice not only by increasing the number of tumor‐specific CD8
+
T cells but also by altering the T cell repertoire, favoring the activation of cells recognizing the mutated P1E/K
d
antigen. CD4
+
T cells triggered the migration of CXCR3
+
CD8
+
T cells by inducing the expression of chemokines in the tumor bed. |
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ISSN: | 0020-7136 1097-0215 |
DOI: | 10.1002/ijc.28617 |