Synthesis of mono-6-deoxy-6-N,N,N′,N′,N′-pentamethylethylenediammonio-cyclomaltoheptaose, a single-isomer, monosubstituted, permanently dicationic β-CD and its use for enantiomer separations by CE

The dichloride salt of mono‐6‐deoxy‐6‐N,N,N′,N′,N′‐pentamethylethylenediammonio‐cyclomaltoheptaose (PEMEDA‐BCD), the first single‐isomer, monosubstituted, permanently dicationic β‐CD has been synthesized, analytically characterized, and used for the capillary electrophoretic separation of the enantiomers of a group of analytes in acidic and basic BGEs. When the concentration of PEMEDA‐BCD was changed in the BGEs, the resulting effective mobilities of the analytes and the respective separation selectivities followed the predictions of the ionic strength‐corrected charged resolving agent migration model. Good separation selectivities and favorable normalized EOF mobilities allowed for the rapid, efficient separation of the enantiomers of anionic, weak acid and nonionic analytes in the low‐ and/or high‐pH BGEs.