Two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimensin (IPG-Dalt): The state of the art and the controversy of vertical versus horizontal systems

After having established the basic protocol of two‐dimensional electrophoresis with immobilized pH gradients in the first dimension (IPG‐Dalt) in 1988 (A. Görg et al., Electrophoresis 1988, 9, 531–546), some critical parameters of the actual IPG‐Dalt protocols as well as the results obtained with horizontal and vertical second‐dimensional sodium dodecyl sulfate‐electrophoresis are demonstrated and discussed.