Rapid mechanical versus conventional acid fixation in electrophoresis

Rapid mechanical versus conventional acid fixation in electrophoresis

We have devised methodology which allows for the rapid mechanical fixation of proteins in polyacrylamide slab gels. Proteins so fixed remain in the gel even after four days of soaking. The potential utility of this approach is discussed.

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Electrophoresis 1985, Vol.6 (4), p.191-192
Hauptverfasser: Gersten, Douglas M., Zapolski, Edward J., Ledley, Robert S.
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:We have devised methodology which allows for the rapid mechanical fixation of proteins in polyacrylamide slab gels. Proteins so fixed remain in the gel even after four days of soaking. The potential utility of this approach is discussed.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.1150060411