Amperometric Detection of Creatinine in Clinical Samples Based on Gold Electrode Arrays Fabricated Using Printed Circuit Board Technology
Herein we report a reliable and stable biosensor for the amperometric detection of creatinine through the ammonium produced in its reaction with creatinine deiminase linked to a Nafion®/polyaniline gold coated surface. The gold electrode array was produced using a low cost printed board circuit tech...
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Veröffentlicht in: | Electroanalysis (New York, N.Y.) N.Y.), 2020-12, Vol.32 (12), p.3054-3059 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Herein we report a reliable and stable biosensor for the amperometric detection of creatinine through the ammonium produced in its reaction with creatinine deiminase linked to a Nafion®/polyaniline gold coated surface. The gold electrode array was produced using a low cost printed board circuit technology. A linear range of 1–1000 μM was obtained with standard deviations of less than 5 % (n=3) obtained. Stability studies were carried out and 80 % activity was maintained after one month. The sensors were tested with 142 serum samples from patients with renal dysfunction at different stage of the disease and validated using a commercial colorimetric method. |
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ISSN: | 1040-0397 1521-4109 |
DOI: | 10.1002/elan.202060446 |