Alcoholysis of waste fats with 2-ethyl-1-hexanol using Candida antarctica lipase A in large-scale tests

Commercial native lipase A from Candida antarctica was used to produce alkyl esters through the alcoholysis of (waste) fats with 2‐ethyl‐1‐hexanol. The process was carried out in batch stirred tank reactors (from 100 mL up to 3000 L). The content of alkyl esters in reaction mixtures was determined by gradient HPLC using an evaporative light scattering detector and the reaction progress was controlled by determining the ratio of the palmitic acid ester peak area to the oleic acid ester peak area in HPLC chromatograms. The results show that alcoholysis is the favoured reaction in presence of excess water and water‐insoluble alcohols in comparison with hydrolysis (fatty acid content