Direct PCR of Intact Bacteria (Colony PCR)

This protocol describes an efficient method for screening intact bacteria for the presence of desired DNA sequences using the polymerase chain reaction (PCR). This method is commonly referred to as colony PCR. © 2016 by John Wiley & Sons, Inc.

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Bibliographische Detailangaben
Veröffentlicht in:	Current protocols in microbiology (Online) 2016-08, Vol.42 (1), p.A.3D.1-A.3D.7
Hauptverfasser:	Woodman, Michael E., Savage, Christina R., Arnold, William K., Stevenson, Brian
Format:	Artikel
Sprache:	eng
Schlagworte:	bacteria Bacteria - classification Bacteria - genetics Bacteria - isolation & purification colony PCR DNA, Bacterial - genetics Polymerase Chain Reaction - methods screen
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	This protocol describes an efficient method for screening intact bacteria for the presence of desired DNA sequences using the polymerase chain reaction (PCR). This method is commonly referred to as colony PCR. © 2016 by John Wiley & Sons, Inc.
ISSN:	1934-8525 1934-8533
DOI:	10.1002/cpmc.14