Attachment of Proteins to Surfaces by Fluorous-Fluorous Interactions Restoring Their Structure and Activity

Attachment of Proteins to Surfaces by Fluorous-Fluorous Interactions Restoring Their Structure and Activity

Above the fold: An innovative strategy using a multifunctional linker system for the specific noncovalent immobilization of proteins to glass surfaces through fluorous–fluorous interactions without causing significant denaturation has been achieved. The principle was demonstrated for the enzymes RNa...

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Veröffentlicht in:ChemPlusChem (Weinheim, Germany) Germany), 2012-12, Vol.77 (12), p.1066-1070
Hauptverfasser: Rapp, Hartmut M., Bacher, Stefanie, Ahrens, Anika, Rapp, Wolfgang, Kammerer, Bernd, Nienhaus, G. Ulrich, Bannwarth, Willi
Format: Artikel
Sprache:eng
Schlagworte:
fluorous-fluorous interactions
FRET
protein immobilization
rcHRP
RNase H
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Zusammenfassung:Above the fold: An innovative strategy using a multifunctional linker system for the specific noncovalent immobilization of proteins to glass surfaces through fluorous–fluorous interactions without causing significant denaturation has been achieved. The principle was demonstrated for the enzymes RNase H and recombinant horseradish peroxidase (see figure).
ISSN:2192-6506
2192-6506
DOI:10.1002/cplu.201200234