Determination of Quinolones and Nonsteroidal anti-Inflammatory Agents in Animal Tissues and Bovine Milk by Microwave-assisted Extraction High Performance Liquid Chromatography

A rapid, specific microwave‐assisted extraction high performance liquid chromatography is described for assaying three quinolones (fleroxacin, lomefloxacin and sparfloxacin) and two nonsteroidal anti‐inflammatory agents (ketoprofen and ibuprofen) in samples of sheep liver, bovine muscle and milk. The optimal microwave‐assisted extraction conditions such as extraction temperature (40 °C), extraction time (6 min), solvent volume (10 mL) and solvent (acetonitrile) were determined by an orthogonal experiment. Recoveries were 60.0% –107% in the concentration range 0.25–0.75 µg·g−1 with good precision (