Sulfonyl Fluoride‐Based Prosthetic Compounds as Potential 18 F Labelling Agents

Nucleophilic incorporation of [ 18 F]F − under aqueous conditions holds several advantages in radiopharmaceutical development, especially with the advent of complex biological pharmacophores. Sulfonyl fluorides can be prepared in water at room temperature, yet they have not been assayed as a potential means to 18 F‐labelled biomarkers for PET chemistry. We developed a general route to prepare bifunctional 4‐formyl‐, 3‐formyl‐, 4‐maleimido‐ and 4‐oxylalkynl‐arylsulfonyl [ 18 F]fluorides from their sulfonyl chloride analogues in 1:1 mixtures of acetonitrile, THF, or t BuOH and Cs[ 18 F]F/Cs 2 CO 3(aq.) in a reaction time of 15 min at room temperature. With the exception of 4‐ N ‐maleimide‐benzenesulfonyl fluoride ( 3 ), pyridine could be used to simplify radiotracer purification by selectively degrading the precursor without significantly affecting observed yields. The addition of pyridine at the start of [ 18 F]fluorination (1:1:0.8 t BuOH/Cs 2 CO 3(aq.) /pyridine) did not negatively affect yields of 3‐formyl‐2,4,6‐trimethylbenzenesulfonyl [ 18 F]fluoride ( 2 ) and dramatically improved the yields of 4‐(prop‐2‐ynyloxy)benzenesulfonyl [ 18 F]fluoride ( 4 ). The N ‐arylsulfonyl‐4‐dimethylaminopyridinium derivative of 4 ( 14 ) can be prepared and incorporates 18 F efficiently in solutions of 100 % aqueous Cs 2 CO 3 (10 mg mL −1 ). As proof‐of‐principle, [ 18 F] 2 was synthesised in a preparative fashion [88(±8) % decay corrected ( n =6) from start‐of‐synthesis] and used to radioactively label an oxyamino‐modified bombesin(6–14) analogue [35(±6) % decay corrected ( n =4) from start‐of‐synthesis]. Total preparation time was 105–109 min from start‐of‐synthesis. Although the 18 F‐peptide exhibited evidence of proteolytic defluorination and modification, our study is the first step in developing an aqueous, room temperature 18 F labelling strategy.