Improved Fitness of Arabidopsis thaliana Nitrilase 2

Improved Fitness of Arabidopsis thaliana Nitrilase 2

A matter of fitness: An Arabidopsis thaliana nitrilase 2 (NIT2) variant with improved specific activity is generated by error prone PCR followed by recombination of advantageous mutations. The improvement is shown not only for the test substrate phenylacetonitrile but also for a diverse set of aroma...

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Veröffentlicht in:ChemCatChem 2010-03, Vol.2 (3), p.263-267
Hauptverfasser: Schreiner, Ulrike, Steinkellner, Georg, Rozzell, J. David, Glieder, Anton, Winkler, Margit
Format: Artikel
Sprache:eng
Schlagworte:
biocatalysis
directed evolution
enzyme catalysis
enzymes
mutation
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Zusammenfassung:A matter of fitness: An Arabidopsis thaliana nitrilase 2 (NIT2) variant with improved specific activity is generated by error prone PCR followed by recombination of advantageous mutations. The improvement is shown not only for the test substrate phenylacetonitrile but also for a diverse set of aromatic nitriles, dicyanides, and other functionalized nitriles.
ISSN:1867-3880
1867-3899
DOI:10.1002/cctc.200900212