Cover Feature: Site‐Specific Antibody Fragment Conjugates for Reversible Staining in Fluorescence Microscopy (7/2021)

To get one step closer to making fluorescence microscopy as easy as filling out shapes in a children's coloring book, we have developed a novel strategy for generating DNA‐ and PNA‐based protein–oligonucleotide conjugates. We use a chemo‐enzymatic, click chemistry–based approach via the TubTag...

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Veröffentlicht in:Chembiochem : a European journal of chemical biology 2021-04, Vol.22 (7), p.1112-1112
Hauptverfasser: Schwach, Jonathan, Kolobynina, Ksenia, Brandstetter, Katharina, Gerlach, Marcus, Ochtrop, Philipp, Helma, Jonas, Hackenberger, Christian P. R., Harz, Hartmann, Cardoso, M. Cristina, Leonhardt, Heinrich, Stengl, Andreas
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container_end_page 1112
container_issue 7
container_start_page 1112
container_title Chembiochem : a European journal of chemical biology
container_volume 22
creator Schwach, Jonathan
Kolobynina, Ksenia
Brandstetter, Katharina
Gerlach, Marcus
Ochtrop, Philipp
Helma, Jonas
Hackenberger, Christian P. R.
Harz, Hartmann
Cardoso, M. Cristina
Leonhardt, Heinrich
Stengl, Andreas
description To get one step closer to making fluorescence microscopy as easy as filling out shapes in a children's coloring book, we have developed a novel strategy for generating DNA‐ and PNA‐based protein–oligonucleotide conjugates. We use a chemo‐enzymatic, click chemistry–based approach via the TubTag to generate our conjugates as depicted in the magnifying glass. Those proteins can readily be used to “paint” our cell for fluorescence microscopy. More information can be found in the communication by J. Schwach, A. Stengl et al. Cover design: J. Schwach.
doi_str_mv 10.1002/cbic.202100115
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source Wiley Online Library - AutoHoldings Journals
subjects antibody conjugates
immunofluorescence staining
nanobodies
peptide nucleic acids
super-resolution microscopy
Tub-tag labelling
title Cover Feature: Site‐Specific Antibody Fragment Conjugates for Reversible Staining in Fluorescence Microscopy (7/2021)
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