Cover Feature: Site‐Specific Antibody Fragment Conjugates for Reversible Staining in Fluorescence Microscopy (7/2021)
To get one step closer to making fluorescence microscopy as easy as filling out shapes in a children's coloring book, we have developed a novel strategy for generating DNA‐ and PNA‐based protein–oligonucleotide conjugates. We use a chemo‐enzymatic, click chemistry–based approach via the TubTag...
Gespeichert in:
Veröffentlicht in: | Chembiochem : a European journal of chemical biology 2021-04, Vol.22 (7), p.1112-1112 |
---|---|
Hauptverfasser: | , , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 1112 |
---|---|
container_issue | 7 |
container_start_page | 1112 |
container_title | Chembiochem : a European journal of chemical biology |
container_volume | 22 |
creator | Schwach, Jonathan Kolobynina, Ksenia Brandstetter, Katharina Gerlach, Marcus Ochtrop, Philipp Helma, Jonas Hackenberger, Christian P. R. Harz, Hartmann Cardoso, M. Cristina Leonhardt, Heinrich Stengl, Andreas |
description | To get one step closer to making fluorescence microscopy as easy as filling out shapes in a children's coloring book, we have developed a novel strategy for generating DNA‐ and PNA‐based protein–oligonucleotide conjugates. We use a chemo‐enzymatic, click chemistry–based approach via the TubTag to generate our conjugates as depicted in the magnifying glass. Those proteins can readily be used to “paint” our cell for fluorescence microscopy. More information can be found in the communication by J. Schwach, A. Stengl et al. Cover design: J. Schwach. |
doi_str_mv | 10.1002/cbic.202100115 |
format | Article |
fullrecord | <record><control><sourceid>wiley_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1002_cbic_202100115</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CBIC202100115</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1295-d7c608e98b75412b37cff26bd6ba1e7b20cf71adcb740180cf49219064aa778b3</originalsourceid><addsrcrecordid>eNqFkLFOwzAQhi0EEqWwMnuEIantpHHCViIClYqQKMyR7ZwrV2lc2SlVNh6BZ-RJSNQKRqa7X7rv9OtD6JqSkBLCJkoaFTLC-kDp9ASNaBxlAU-i6PS4x4zxc3Th_ZoQkiURHaF9bj_A4QJEu3Nwh5emhe_Pr-UWlNFG4VnTGmmrDhdOrDbQtDi3zXq3Ei14rK3Dr9Dz3sga8LIVpjHNCpsGF_XOOvAKGgX42ShnvbLbDt_wyVDx9hKdaVF7uDrOMXovHt7yp2Dx8jjPZ4tAUZZNg4qrhKSQpZJPY8pkxJXWLJFVIgUFLhlRmlNRKcljQtM-xRmjGUliIThPZTRG4eHv0MA70OXWmY1wXUlJOWgrB23lr7YeyA7A3tTQ_XNd5vfz_I_9AXx0cro</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Cover Feature: Site‐Specific Antibody Fragment Conjugates for Reversible Staining in Fluorescence Microscopy (7/2021)</title><source>Wiley Online Library - AutoHoldings Journals</source><creator>Schwach, Jonathan ; Kolobynina, Ksenia ; Brandstetter, Katharina ; Gerlach, Marcus ; Ochtrop, Philipp ; Helma, Jonas ; Hackenberger, Christian P. R. ; Harz, Hartmann ; Cardoso, M. Cristina ; Leonhardt, Heinrich ; Stengl, Andreas</creator><creatorcontrib>Schwach, Jonathan ; Kolobynina, Ksenia ; Brandstetter, Katharina ; Gerlach, Marcus ; Ochtrop, Philipp ; Helma, Jonas ; Hackenberger, Christian P. R. ; Harz, Hartmann ; Cardoso, M. Cristina ; Leonhardt, Heinrich ; Stengl, Andreas</creatorcontrib><description>To get one step closer to making fluorescence microscopy as easy as filling out shapes in a children's coloring book, we have developed a novel strategy for generating DNA‐ and PNA‐based protein–oligonucleotide conjugates. We use a chemo‐enzymatic, click chemistry–based approach via the TubTag to generate our conjugates as depicted in the magnifying glass. Those proteins can readily be used to “paint” our cell for fluorescence microscopy. More information can be found in the communication by J. Schwach, A. Stengl et al. Cover design: J. Schwach.</description><identifier>ISSN: 1439-4227</identifier><identifier>EISSN: 1439-7633</identifier><identifier>DOI: 10.1002/cbic.202100115</identifier><language>eng</language><subject>antibody conjugates ; immunofluorescence staining ; nanobodies ; peptide nucleic acids ; super-resolution microscopy ; Tub-tag labelling</subject><ispartof>Chembiochem : a European journal of chemical biology, 2021-04, Vol.22 (7), p.1112-1112</ispartof><rights>2021 Wiley‐VCH GmbH</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0001-8427-8859 ; 0000-0003-4498-3136 ; 0000-0003-1218-2107 ; 0000-0001-6158-452X ; 0000-0002-2109-5585 ; 0000-0002-8719-2943 ; 0000-0002-8035-8867 ; 0000-0001-7457-4742 ; 0000-0002-8802-3260</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcbic.202100115$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcbic.202100115$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,782,786,1419,27931,27932,45581,45582</link.rule.ids></links><search><creatorcontrib>Schwach, Jonathan</creatorcontrib><creatorcontrib>Kolobynina, Ksenia</creatorcontrib><creatorcontrib>Brandstetter, Katharina</creatorcontrib><creatorcontrib>Gerlach, Marcus</creatorcontrib><creatorcontrib>Ochtrop, Philipp</creatorcontrib><creatorcontrib>Helma, Jonas</creatorcontrib><creatorcontrib>Hackenberger, Christian P. R.</creatorcontrib><creatorcontrib>Harz, Hartmann</creatorcontrib><creatorcontrib>Cardoso, M. Cristina</creatorcontrib><creatorcontrib>Leonhardt, Heinrich</creatorcontrib><creatorcontrib>Stengl, Andreas</creatorcontrib><title>Cover Feature: Site‐Specific Antibody Fragment Conjugates for Reversible Staining in Fluorescence Microscopy (7/2021)</title><title>Chembiochem : a European journal of chemical biology</title><description>To get one step closer to making fluorescence microscopy as easy as filling out shapes in a children's coloring book, we have developed a novel strategy for generating DNA‐ and PNA‐based protein–oligonucleotide conjugates. We use a chemo‐enzymatic, click chemistry–based approach via the TubTag to generate our conjugates as depicted in the magnifying glass. Those proteins can readily be used to “paint” our cell for fluorescence microscopy. More information can be found in the communication by J. Schwach, A. Stengl et al. Cover design: J. Schwach.</description><subject>antibody conjugates</subject><subject>immunofluorescence staining</subject><subject>nanobodies</subject><subject>peptide nucleic acids</subject><subject>super-resolution microscopy</subject><subject>Tub-tag labelling</subject><issn>1439-4227</issn><issn>1439-7633</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNqFkLFOwzAQhi0EEqWwMnuEIantpHHCViIClYqQKMyR7ZwrV2lc2SlVNh6BZ-RJSNQKRqa7X7rv9OtD6JqSkBLCJkoaFTLC-kDp9ASNaBxlAU-i6PS4x4zxc3Th_ZoQkiURHaF9bj_A4QJEu3Nwh5emhe_Pr-UWlNFG4VnTGmmrDhdOrDbQtDi3zXq3Ei14rK3Dr9Dz3sga8LIVpjHNCpsGF_XOOvAKGgX42ShnvbLbDt_wyVDx9hKdaVF7uDrOMXovHt7yp2Dx8jjPZ4tAUZZNg4qrhKSQpZJPY8pkxJXWLJFVIgUFLhlRmlNRKcljQtM-xRmjGUliIThPZTRG4eHv0MA70OXWmY1wXUlJOWgrB23lr7YeyA7A3tTQ_XNd5vfz_I_9AXx0cro</recordid><startdate>20210406</startdate><enddate>20210406</enddate><creator>Schwach, Jonathan</creator><creator>Kolobynina, Ksenia</creator><creator>Brandstetter, Katharina</creator><creator>Gerlach, Marcus</creator><creator>Ochtrop, Philipp</creator><creator>Helma, Jonas</creator><creator>Hackenberger, Christian P. R.</creator><creator>Harz, Hartmann</creator><creator>Cardoso, M. Cristina</creator><creator>Leonhardt, Heinrich</creator><creator>Stengl, Andreas</creator><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0001-8427-8859</orcidid><orcidid>https://orcid.org/0000-0003-4498-3136</orcidid><orcidid>https://orcid.org/0000-0003-1218-2107</orcidid><orcidid>https://orcid.org/0000-0001-6158-452X</orcidid><orcidid>https://orcid.org/0000-0002-2109-5585</orcidid><orcidid>https://orcid.org/0000-0002-8719-2943</orcidid><orcidid>https://orcid.org/0000-0002-8035-8867</orcidid><orcidid>https://orcid.org/0000-0001-7457-4742</orcidid><orcidid>https://orcid.org/0000-0002-8802-3260</orcidid></search><sort><creationdate>20210406</creationdate><title>Cover Feature: Site‐Specific Antibody Fragment Conjugates for Reversible Staining in Fluorescence Microscopy (7/2021)</title><author>Schwach, Jonathan ; Kolobynina, Ksenia ; Brandstetter, Katharina ; Gerlach, Marcus ; Ochtrop, Philipp ; Helma, Jonas ; Hackenberger, Christian P. R. ; Harz, Hartmann ; Cardoso, M. Cristina ; Leonhardt, Heinrich ; Stengl, Andreas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1295-d7c608e98b75412b37cff26bd6ba1e7b20cf71adcb740180cf49219064aa778b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>antibody conjugates</topic><topic>immunofluorescence staining</topic><topic>nanobodies</topic><topic>peptide nucleic acids</topic><topic>super-resolution microscopy</topic><topic>Tub-tag labelling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schwach, Jonathan</creatorcontrib><creatorcontrib>Kolobynina, Ksenia</creatorcontrib><creatorcontrib>Brandstetter, Katharina</creatorcontrib><creatorcontrib>Gerlach, Marcus</creatorcontrib><creatorcontrib>Ochtrop, Philipp</creatorcontrib><creatorcontrib>Helma, Jonas</creatorcontrib><creatorcontrib>Hackenberger, Christian P. R.</creatorcontrib><creatorcontrib>Harz, Hartmann</creatorcontrib><creatorcontrib>Cardoso, M. Cristina</creatorcontrib><creatorcontrib>Leonhardt, Heinrich</creatorcontrib><creatorcontrib>Stengl, Andreas</creatorcontrib><collection>CrossRef</collection><jtitle>Chembiochem : a European journal of chemical biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schwach, Jonathan</au><au>Kolobynina, Ksenia</au><au>Brandstetter, Katharina</au><au>Gerlach, Marcus</au><au>Ochtrop, Philipp</au><au>Helma, Jonas</au><au>Hackenberger, Christian P. R.</au><au>Harz, Hartmann</au><au>Cardoso, M. Cristina</au><au>Leonhardt, Heinrich</au><au>Stengl, Andreas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cover Feature: Site‐Specific Antibody Fragment Conjugates for Reversible Staining in Fluorescence Microscopy (7/2021)</atitle><jtitle>Chembiochem : a European journal of chemical biology</jtitle><date>2021-04-06</date><risdate>2021</risdate><volume>22</volume><issue>7</issue><spage>1112</spage><epage>1112</epage><pages>1112-1112</pages><issn>1439-4227</issn><eissn>1439-7633</eissn><abstract>To get one step closer to making fluorescence microscopy as easy as filling out shapes in a children's coloring book, we have developed a novel strategy for generating DNA‐ and PNA‐based protein–oligonucleotide conjugates. We use a chemo‐enzymatic, click chemistry–based approach via the TubTag to generate our conjugates as depicted in the magnifying glass. Those proteins can readily be used to “paint” our cell for fluorescence microscopy. More information can be found in the communication by J. Schwach, A. Stengl et al. Cover design: J. Schwach.</abstract><doi>10.1002/cbic.202100115</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-8427-8859</orcidid><orcidid>https://orcid.org/0000-0003-4498-3136</orcidid><orcidid>https://orcid.org/0000-0003-1218-2107</orcidid><orcidid>https://orcid.org/0000-0001-6158-452X</orcidid><orcidid>https://orcid.org/0000-0002-2109-5585</orcidid><orcidid>https://orcid.org/0000-0002-8719-2943</orcidid><orcidid>https://orcid.org/0000-0002-8035-8867</orcidid><orcidid>https://orcid.org/0000-0001-7457-4742</orcidid><orcidid>https://orcid.org/0000-0002-8802-3260</orcidid><oa>free_for_read</oa></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1439-4227 |
ispartof | Chembiochem : a European journal of chemical biology, 2021-04, Vol.22 (7), p.1112-1112 |
issn | 1439-4227 1439-7633 |
language | eng |
recordid | cdi_crossref_primary_10_1002_cbic_202100115 |
source | Wiley Online Library - AutoHoldings Journals |
subjects | antibody conjugates immunofluorescence staining nanobodies peptide nucleic acids super-resolution microscopy Tub-tag labelling |
title | Cover Feature: Site‐Specific Antibody Fragment Conjugates for Reversible Staining in Fluorescence Microscopy (7/2021) |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-04T01%3A58%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-wiley_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cover%20Feature:%20Site%E2%80%90Specific%20Antibody%20Fragment%20Conjugates%20for%20Reversible%20Staining%20in%20Fluorescence%20Microscopy%20(7/2021)&rft.jtitle=Chembiochem%20:%20a%20European%20journal%20of%20chemical%20biology&rft.au=Schwach,%20Jonathan&rft.date=2021-04-06&rft.volume=22&rft.issue=7&rft.spage=1112&rft.epage=1112&rft.pages=1112-1112&rft.issn=1439-4227&rft.eissn=1439-7633&rft_id=info:doi/10.1002/cbic.202100115&rft_dat=%3Cwiley_cross%3ECBIC202100115%3C/wiley_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |