Trifluoroacetyl‐effect on amino‐single benzene‐based fluorophores: Synthesis, optical properties, and cytotoxicity

Amino‐single benzene‐based fluorophores (SBBFs) are representative small‐size fluorescent dyes that have an electron push‐pull structure within the benzene core. In this work, a new library of amino‐SBBFs, which have trifluoroacetyl moiety at the electron‐pushing amine group (named SBBF‐TFA), featuring a variety of methylamines, dimethylamines, as well as acetyl. To explore the SBBF‐TFA library, we conducted (i) library synthesis, (ii) assessment of various photophysical properties in nine different types of organic/aqueous solvents, and (iii) evaluation of cytotoxicity in the HeLa cell line. We believe that our findings provide valuable insights for advancing novel small‐molecule fluorescent probes and materials with diverse applications across pharmacochemical fields. We disclosed a new library based on amino‐single benzene‐based fluorophore (SBBF) with substituting trifluoroacetyl moiety. The synthesis, optical properties in various organic/aqueous solvents, and evaluated cell viability were systematically conducted.