Isolation of a respiratory-deficient Kluyveromyces fragilis mutant for the production of ethanol from Jerusalem artichoke

A respiratory‐deficient, mutant of Kluyveromyces fragilis was isolated using a ethidium bromide mutagenesis. It was characterized by a loss of cytochromes a + a3 and by an improvement of its inulinase activity. Under anaerobic conditions this mutant was always better than the wild strain for ethanol...

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Veröffentlicht in:	Biotechnol. Bioeng.; (United States) 1987-05, Vol.29 (7), p.850-858
Hauptverfasser:	Guiraud, J.P, Bourgi, J, Stervinou, M, Claisse, M, Galzy, P
Format:	Artikel
Sprache:	eng
Schlagworte:	09 BIOMASS FUELS 090222 - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989) Agronomy. Soil science and plant productions ALCOHOLES ALCOHOLS ALCOOL ANAEROBIC CONDITIONS BIOCONVERSION Biological and medical sciences Biotechnology CARBOHYDRATES ENZYME ACTIVITY ENZYMES ETHANOL FERMENTATION FOOD Food industries FRUCTOSE Fundamental and applied biological sciences. Psychology FUNGI General agronomy. Plant production HELIANTHUS TUBEROSUS HEXOSES HYDROLASES HYDROXY COMPOUNDS INULIN JERUSALEM ARTICHOKES KETONES KLUYVEROMYCES FRAGILIS Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology MICROORGANISMS MONOSACCHARIDES MUTANT MUTANTES MUTANTS ORGANIC COMPOUNDS PEPTIDE HYDROLASES PLANTS POLYSACCHARIDES PRODUCCION PRODUCTION SACCHARIDES Use and upgrading of agricultural and food by-products. Biotechnology Use of agricultural and forest wastes. Biomass use, bioconversion VEGETABLES YEASTS YIELDS
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Beschreibung
Zusammenfassung:	A respiratory‐deficient, mutant of Kluyveromyces fragilis was isolated using a ethidium bromide mutagenesis. It was characterized by a loss of cytochromes a + a3 and by an improvement of its inulinase activity. Under anaerobic conditions this mutant was always better than the wild strain for ethanol production especially from Jerusalem artichoke extracts containing large amounts of high polyfructosans ("early" extracts).
ISSN:	0006-3592 1097-0290
DOI:	10.1002/bit.260290707