Hydroxylamine as a competitive inhibitor for the kinetic enzymatic assay of ethanol in relatively concentrated solutions

The kinetic enzymatic assay of ethanol may be optimized for a selected range of substrate concentrations by a judicious choice of acetaldehyde trapping agent and ADH competitive inhibitor. Hydrazine and hydroxylamine can serve both of these functions. If the two agents were used simultaneously, hydrazine would serve as the more potent trapping agent and hydroxylamine as the more potent competitive inhibitor.