The Determination of Axis Lengths of Dunaliella from the Image Analysis of TEM Sections through Randomly Orientated Cells

We present a method which improves the determination of geometric parameters of unicellular organisms with prolate ellipsoid geometry, such as Dunaliella. Approximately determined axis lenghts were obtained from transmission electron micrographs of ultrathin sections of microalgae. The density funct...

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Veröffentlicht in:	Biometrical journal 1996, Vol.38 (8), p.907-920
Hauptverfasser:	Roessler, Jochen, Jones, Tim, Bérubé, Kelly Ann
Format:	Artikel
Sprache:	eng
Schlagworte:	Algae Applications Applied sciences Biology, psychology, social sciences Ellipsoids Exact sciences and technology Image Analysis Image processing Information, signal and communications theory Insurance, economics, finance Mathematics Medical sciences Parametric inference Probability and statistics Reliability, life testing, quality control Sciences and techniques of general use Signal processing Statistics Stereology Telecommunications and information theory Transmission Electron Microscopy
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Beschreibung
Zusammenfassung:	We present a method which improves the determination of geometric parameters of unicellular organisms with prolate ellipsoid geometry, such as Dunaliella. Approximately determined axis lenghts were obtained from transmission electron micrographs of ultrathin sections of microalgae. The density functions generated by these ‘axis lengths’ have poles which can be used to determine the short half axis length and ratio of axis lengths of the ellipsoid in order to determine the true cell volume.
ISSN:	0323-3847 1521-4036
DOI:	10.1002/bimj.4710380803