Analysis of differentially expressed proteins in late‐stationary growth phase of Mycobacterium tuberculosis H 37 R v

Mycobacterium tuberculosis is a causative agent of tuberculosis ( TB ). The ability of M. tuberculosis to be quiescent in the cell has caused the emergence of latent infection. A comprehensive proteomic analysis of M. tuberculosis H 37 R v over three growth phases, namely mid‐log (14‐day culture), e...

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Veröffentlicht in:Biotechnology and applied biochemistry 2014-03, Vol.61 (2), p.153-164
Hauptverfasser: Ang, Kai‐Cheen, Ibrahim, Pazilah, Gam, Lay‐Harn
Format: Artikel
Sprache:eng
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Zusammenfassung:Mycobacterium tuberculosis is a causative agent of tuberculosis ( TB ). The ability of M. tuberculosis to be quiescent in the cell has caused the emergence of latent infection. A comprehensive proteomic analysis of M. tuberculosis H 37 R v over three growth phases, namely mid‐log (14‐day culture), early stationary (28‐day culture), and late stationary (50‐day culture), was performed in order to study the change in proteome from the mid‐log phase to late‐stationary phase. Combination methods of two‐dimensional electrophoresis (2‐ DE ) and tandem mass spectrometry were used to generate proteome maps of M. tuberculosis at different growth phases. Ten proteins were detected differentially expressed in the late‐stationary phase compared with the other two phases. These proteins were SucD, TrpD, and Rv2161c, which belong to metabolic pathway proteins; FadE5, AccD5, DesA1, and Rv1139c are proteins involved in cell wall or lipid biosynthesis, whereas TB21.7 and Rv3224 are conserved hypothetical proteins with unknown function. A surface antigen protein, DesA1, was not detectable in the late‐stationary phase, although present in both log and early‐stationary phases. The changes in the expression levels of these proteins were in line with the growth environment changes of the bacteria from mid‐log phase to late‐stationary phase. The information gathered may be valuable in the intervention against latent TB infection.
ISSN:0885-4513
1470-8744
DOI:10.1002/bab.1137