Influence of pentaerythritol triacrylate acrylic carrier swelling on immobilization of enzymes

Suspension copolymerization and modification of pentaerythritol triacrylate (PENTA)–butyl acrylate (BA) matrices with ethylenediamine (EtDA) are discussed. It is shown that aminolysis in water solution offers gel structure carriers with small pore radii (about 3 nm). These carriers are characterized by the high swelling and sharp correlation between the pH value and the specific volume. The enzymes (acylase, glucoamylase, and peroxydase) are immobilized by means of the glutaraldehyde method. The best matrix seems to be copolymers modified in a 95% solution of ethylenediamine in water. Preparations do not lose activity after 1 month storage at 4°C. © 1995 John Wiley & Sons, Inc.