Solubilization of calfskin collagen in citrate buffer with the use of automated equipment
An automated collagen solubilizing apparatus that maintains constant electrolytic conditions during solubilization is described. It was used in solubilization of 6.95% of a calfskin corium as monomeric collagen in citrate buffer at pH 3.44 and ionic strength of 0.44 and a temperature of 8°C. It is s...
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| Veröffentlicht in: | Journal of applied polymer science 1969-01, Vol.13 (1), p.141-148 |
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| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
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| Zusammenfassung: | An automated collagen solubilizing apparatus that maintains constant electrolytic conditions during solubilization is described. It was used in solubilization of 6.95% of a calfskin corium as monomeric collagen in citrate buffer at pH 3.44 and ionic strength of 0.44 and a temperature of 8°C. It is shown that this is the limit of solubilization for undenatured collagen in this buffer. |
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| ISSN: | 0021-8995 1097-4628 |
| DOI: | 10.1002/app.1969.070130115 |