Die nutzung gentechnischer methoden zur produktion wertvoller proteine in mikroorganismen

The genetic make‐up of living systems especially of isolated cells can be altered by a variety of methods including chemical or light‐induced mutation. More modern procedures are the in vitro recombination of nucleic acids and the cell‐cell fusion. The new methods are especially useful to reprogram microorganisms to produce valuable proteins or other natural products in excess quantties. In the following we will elucidate the importance of the new techniques by describing the bacterial production of glucose dehydrogenase, of the proteinase inhibitor stefin A and by outlining the methods and promisses of substractive cloning. Although the economic value of genetic engineering techniques still awaits justification, the importance of the methodology for basic biological research is well documented.