The effect of phenyltin chlorides on osmotically induced erythrocyte haemolysis

The toxicity of many amphiphilic compounds may result from their effect on the lipid phase of biological membranes. Upon incorporation such compounds may change the properties of membranes in general and in particular alter the organization of membrane lipids. These changes should affect, among other things, the mechanical properties of membranes. We selected two amphiphilic compounds, diphenyltin dichloride (Ph2SnCl2) and triphenyltin chloride (Ph3SnCl), which are known to be located at different regions of the lipid bilayer and to be toxic. As a model biological membrane the erythrocyte plasma membrane was used. Analysis of the haemolysis kinetics showed differences between the effect of the compound studied on mechanical properties at so‐called non‐lytic concentrations. Diphenyltin dichloride showed a limited effect on erythrocyte haemolysis, whereas triphenyltin chloride affected all the parameters measured (extent of initial haemolysis, extent of final haemolysis and membrane mechanical strength). We correlated these effects with the location of the investigated compounds in liposomes. The presented data show that triphenyltin chloride reduces the erythrocyte plasma membrane mechanical strength and increases the extent of haemolysis under osmotic stress conditions. Copyright © 2005 John Wiley & Sons, Ltd. The analysis of the haemolysis kinetics of human erythocytes in the presence of diphenyltin dichloride (A) and triphenyltin chloride (B) shows differences between the effect of the compound studied on mechanical properties at the so‐called non‐lytic concentrations. Diphenyltin dichloride shows a limited effect on erythrocyte haemolysis, whereas triphenyltin chloride affects all the parameters measured (extent of initial haemolysis, extent of final haemolysis and membrane mechanical strength). We correlate these effects with location of the investigated compounds in liposomes.