Bottom-Up Fabrication of Nanopatterned Polymers on DNA Origami by In Situ Atom-Transfer Radical Polymerization

Bottom‐up strategies to fabricate patterned polymers at the nanoscale represent an emerging field in the development of advanced nanodevices, such as biosensors, nanofluidics, and nanophotonics. DNA origami techniques provide access to distinct architectures of various sizes and shapes and present manifold opportunities for functionalization at the nanoscale with the highest precision. Herein, we conduct in situ atom‐transfer radical polymerization (ATRP) on DNA origami, yielding differently nanopatterned polymers of various heights. After cross‐linking, the grafted polymeric nanostructures can even stably exist in solution without the DNA origami template. This straightforward approach allows for the fabrication of patterned polymers with low nanometer resolution, which provides access to unique DNA‐based functional hybrid materials. Surface‐initiated polymerization reactions on DNA origami enable the precise design of nanopatterned polymers. Characterization by atomic force microscopy, gel electrophoresis, and time‐of‐flight secondary‐ion mass spectrometry showed that this approach can be used to fabricate polymers of different patterns and lengths on the nanoscale.