The Conformation of the Prion Domain of Sup35 p in Isolation and in the Full-Length Protein

The whole is not the sum of the parts: Fibrils form both from the full‐length Sup35 prion protein and also from its isolated NM domain. A conformation analysis of both shows that Sup35NM and fragments thereof, which are often used as convenient models for prion fibril assembly, have a very different conformation of the prion domains.