Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering

Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering

In the loop: Engineering of the surface loop in haloalkane dehalogenases affects their enantiodiscrimination behavior. The temperature dependence of the enantioselectivity (lnE versus 1/T) of β‐bromoalkanes by haloalkane dehalogenases is reversed (red data points) by deletion of the surface loop; th...

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Veröffentlicht in:Angewandte Chemie (International ed.) 2010-08, Vol.49 (35), p.6111-6115
Hauptverfasser: Prokop, Zbynek, Sato, Yukari, Brezovsky, Jan, Mozga, Tomas, Chaloupkova, Radka, Koudelakova, Tana, Jerabek, Petr, Stepankova, Veronika, Natsume, Ryo, van Leeuwen, Jan G.E, Janssen, Dick B, Florian, Jan, Nagata, Yuji, Senda, Toshiya, Damborsky, Jiri
Format: Artikel
Sprache:eng
Schlagworte:
Amino Acid Sequence
Biocatalysis
Bradyrhizobium - enzymology
Catalytic Domain
Crystallography, X-Ray
enantioselectivity
enzymes
haloalkane dehalogenases
Hydrolases - chemistry
Hydrolases - genetics
Hydrolases - metabolism
Kinetics
Protein Engineering
Stereoisomerism
Substrate Specificity
Thermodynamics
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Zusammenfassung:In the loop: Engineering of the surface loop in haloalkane dehalogenases affects their enantiodiscrimination behavior. The temperature dependence of the enantioselectivity (lnE versus 1/T) of β‐bromoalkanes by haloalkane dehalogenases is reversed (red data points) by deletion of the surface loop; the selectivity switches back when an additional single‐point mutation is made. This behavior is not observed for α‐bromoesters.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201001753