Single-Molecule Kinetics of Two-Step Divalent Cation Chelation

Single-Molecule Kinetics of Two-Step Divalent Cation Chelation

A double hug: Two half‐chelating ligands were covalently attached within the lumen of a protein nanopore (see picture). By electrical recording, the formation of fully chelated Zn2+ ions was monitored at the single‐molecule level, thus revealing the rate constants for the eight major steps in the pr...

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Veröffentlicht in:Angewandte Chemie (International ed.) 2010-07, Vol.49 (30), p.5085-5090
Hauptverfasser: Hammerstein, Anne F, Shin, Seong-Ho, Bayley, Hagan
Format: Artikel
Sprache:eng
Schlagworte:
Cations, Divalent - chemistry
Chelating Agents - chemistry
chelating ligands
Hemolysin Proteins - chemistry
Kinetics
Ligands
Models, Molecular
nanoreactors
Protein Structure, Quaternary
single-molecule studies
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Zusammenfassung:A double hug: Two half‐chelating ligands were covalently attached within the lumen of a protein nanopore (see picture). By electrical recording, the formation of fully chelated Zn2+ ions was monitored at the single‐molecule level, thus revealing the rate constants for the eight major steps in the process.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200906601