A Heteronuclear Direct-Detection NMR Spectroscopy Experiment for Protein-Backbone Assignment

A Heteronuclear Direct-Detection NMR Spectroscopy Experiment for Protein-Backbone Assignment

The starting point of any standard protocol for protein‐structure determination by NMR spectroscopy is the sequence‐specific resonance assignment of the polypeptide chain. An experiment for determining backbone assignment starts and ends on 13C nuclei and exploits only heteronuclei. This experiment...

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Veröffentlicht in:Angewandte Chemie International Edition 2004-04, Vol.43 (17), p.2257-2259
Hauptverfasser: Bertini, Ivano, Duma, Luminita, Felli, Isabella C., Fey, Michael, Luchinat, Claudio, Pierattelli, Roberta, Vasos, Paul R.
Format: Artikel
Sprache:eng
Schlagworte:
NMR spectroscopy
protein structures
proteins
structure elucidation
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Zusammenfassung:The starting point of any standard protocol for protein‐structure determination by NMR spectroscopy is the sequence‐specific resonance assignment of the polypeptide chain. An experiment for determining backbone assignment starts and ends on 13C nuclei and exploits only heteronuclei. This experiment paves a new route for those cases in which the 1H lines are too severely broadened.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200453661