Green Fabrication of (6,5)Carbon Nanotube/Protein Transistor Endowed with Specific Recognition

A general single‐step approach is introduced for the green fabrication of hybrid biosensors from water dispersion. The resulting device integrates the semiconducting properties of a carbon nanotube (CNT) and the functionality of a protein. In the initial aqueous phase, the protein (viz., lysozyme [LZ]) disperses the (6,5)CNT. Drop‐casting of the dispersion on a test pattern (a silicon wafer with interdigitated Au source and drain electrodes) yields a fully operating, robust, electrolyte‐gated transistor (EGT) in one step. The EGT response to biorecognition is then assessed using the LZ inhibitor N‐acetyl glucosamine trisaccharide. Analysis of the output signal allows one to extract a protein‐substrate binding constant in line with values reported for the free (without CNT) system. The methodology is robust, easy to optimize, redirectable toward different targets and sets the grounds for a new class of CNT‐protein biosensors that overcome many limitations of the technology of fabrication of CNT biosensors. A general single‐step approach for green fabrication of hybrid biosensors from water dispersion is presented. The device integrates semiconducting carbon nanotube and the functionality of a protein. The device bio‐response is assessed using the protein inhibitor N‐acetyl glucosamine trisaccharide. Analysis of the signal allows extraction of a binding constant in line with the values reported for the free system.