Differential Staining of Bacteria: Acid Fast Stain

Acid‐fastness is an uncommon characteristic shared by the genera Mycobacterium (Section 10A) and Nocardia. Because of this feature, this stain is extremely helpful in identification of these bacteria. Although Gram positive, acid‐fast bacteria do not take the crystal violet into the wall well, appea...

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Veröffentlicht in:	Current protocols in microbiology (Online) 2009-11, Vol.15 (1), p.A.3H.1-A.3H.5
Hauptverfasser:	Reynolds, Jackie, Moyes, Rita B., Breakwell, Donald P.
Format:	Artikel
Sprache:	eng
Schlagworte:	acid‐fast stain bacteria Bacteria - chemistry Bacteria - classification Bacterial Typing Techniques - methods carbol fuchsin Cell Wall - chemistry Coloring Agents - chemistry differential stain Mycobacterium Staining and Labeling - methods
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Acid‐fastness is an uncommon characteristic shared by the genera Mycobacterium (Section 10A) and Nocardia. Because of this feature, this stain is extremely helpful in identification of these bacteria. Although Gram positive, acid‐fast bacteria do not take the crystal violet into the wall well, appearing very light purple rather than the deep purple of normal Gram‐positive bacteria. Curr. Protoc. Microbiol. 15:A.3H.1‐A.3H.5. © 2009 by John Wiley & Sons, Inc.
ISSN:	1934-8525 1934-8533
DOI:	10.1002/9780471729259.mca03hs15