Regulation of BC 200 RNA ‐mediated translation inhibition by hn RNP E1 and E2

The long noncoding RNA BC 200 (brain cytoplasmic RNA , 200 nucleotides) acts as a translational modulator of local protein synthesis at dendrites. BC200 RNA has been shown to inhibit translation in vitro , but it remains unknown how this translation inhibition might be controlled in a cell. Here, we performed yeast three‐hybrid screening and identified hn RNP E1 and hn RNP E2 as BC200 RNA‐interacting proteins. We found that: these hn RNA proteins could restore BC200 RNA‐inhibited translation; BC200 RNA interacts with hn RNP E1 and E2 mainly through its unique 3′ C‐rich domain; and the RNA binding specificities and modes of the two proteins differed somewhat. Our results offer new insights into the regulation of BC200 RNA‐mediated translation inhibition.