Use of the Hemagglutinating Virus of Japan (HVJ) Envelope as a Versatile Delivery System for Nucleic Acids and Proteins to Leukocytes In Vitro

Use of the Hemagglutinating Virus of Japan (HVJ) Envelope as a Versatile Delivery System for Nucleic Acids and Proteins to Leukocytes In Vitro

The hemagglutinating virus of Japan (HVJ; also called the Sendai virus) envelope has been developed as a safe and efficient non‐viral vector. Because replication and transcription of genomic RNA is inactivated by β‐propiolactone treatment or UV‐irradiation, the HVJ envelope is extremely safe. This u...

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Veröffentlicht in:Current Protocols in Immunology 2010-04, Vol.89 (1), p.10.17D.1-10.17D.9
Hauptverfasser: Kondo, Yoshitaka, Miyata, Keizo, Kato, Fuminori
Format: Artikel
Sprache:eng
Schlagworte:
hemagglutinating virus of Japan
Humans
HVJ envelope
Leukocytes - metabolism
Proteins - metabolism
RNA, Small Interfering - metabolism
Sendai virus
Sendai virus - chemistry
siRNA transfection
Transfection - methods
Viral Envelope Proteins - metabolism
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creator Kondo, Yoshitaka
Miyata, Keizo
Kato, Fuminori
description The hemagglutinating virus of Japan (HVJ; also called the Sendai virus) envelope has been developed as a safe and efficient non‐viral vector. Because replication and transcription of genomic RNA is inactivated by β‐propiolactone treatment or UV‐irradiation, the HVJ envelope is extremely safe. This unit describes the method of transfection of siRNA and protein with the HVJ envelope. Curr. Protoc. Immunol. 89:10.17D.1‐10.17D.9. © 2010 by John Wiley & Sons, Inc.
doi_str_mv 10.1002/0471142735.im1017ds89
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subjects hemagglutinating virus of Japan
Humans
HVJ envelope
Leukocytes - metabolism
Proteins - metabolism
RNA, Small Interfering - metabolism
Sendai virus
Sendai virus - chemistry
siRNA transfection
Transfection - methods
Viral Envelope Proteins - metabolism
title Use of the Hemagglutinating Virus of Japan (HVJ) Envelope as a Versatile Delivery System for Nucleic Acids and Proteins to Leukocytes In Vitro
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