Use of the Hemagglutinating Virus of Japan (HVJ) Envelope as a Versatile Delivery System for Nucleic Acids and Proteins to Leukocytes In Vitro

The hemagglutinating virus of Japan (HVJ; also called the Sendai virus) envelope has been developed as a safe and efficient non‐viral vector. Because replication and transcription of genomic RNA is inactivated by β‐propiolactone treatment or UV‐irradiation, the HVJ envelope is extremely safe. This unit describes the method of transfection of siRNA and protein with the HVJ envelope. Curr. Protoc. Immunol. 89:10.17D.1‐10.17D.9. © 2010 by John Wiley & Sons, Inc.