Optical Methods

Equilibrium optical melting curves are used in this unit to determine thermodynamic parameters of nucleic acid complex formation. This section contains all of the relevant equations and a discussion of which are most appropriate to a given situation. Additionally, procedures are given for making pre...

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Veröffentlicht in:	Current Protocols in Nucleic Acid Chemistry 2001-05, Vol.Chapter 7 (1), p.7.3.1-7.3.17
1. Verfasser:	Plum, G. Eric
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry - methods Nucleic Acid Denaturation Oligonucleotides - chemistry Optics and Photonics Phosphates - analysis Reference Standards Thermodynamics
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Equilibrium optical melting curves are used in this unit to determine thermodynamic parameters of nucleic acid complex formation. This section contains all of the relevant equations and a discussion of which are most appropriate to a given situation. Additionally, procedures are given for making preliminary determinations of molar extinction coefficients and for determining the number of oligonucleotides in a complex. The section on extinction coefficients is particularly essential to anyone needing to know solution concentrations.
ISSN:	1934-9270 1934-9289
DOI:	10.1002/0471142700.nc0703s00