Use of Electrophilic Substitution to Form Site‐Specific Cross‐Links in DNA

Use of Electrophilic Substitution to Form Site‐Specific Cross‐Links in DNA

This unit describes the synthesis, purification, and analysis of modified oligodeoxynucleotides containing 4‐thio‐2′‐deoxyuridine, and the use of these modified oligodeoxynucleotides in conjunction with bis‐electrophilic tethers to form site‐specific cross‐links with a complementary DNA target. Thes...

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Veröffentlicht in:Current Protocols in Nucleic Acid Chemistry 2001-09, Vol.6 (1), p.5.5.1-5.5.6
Hauptverfasser: Pires, Richard M., Coleman, Robert S.
Format: Artikel
Sprache:eng
Schlagworte:
Biochemistry - methods
Cross-Linking Reagents - chemistry
DNA - chemistry
Phenylenediamines - chemistry
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Beschreibung
Zusammenfassung:This unit describes the synthesis, purification, and analysis of modified oligodeoxynucleotides containing 4‐thio‐2′‐deoxyuridine, and the use of these modified oligodeoxynucleotides in conjunction with bis‐electrophilic tethers to form site‐specific cross‐links with a complementary DNA target. These modified oligodeoxynucleotides provide a flexible tool for studies directed at biophysical investigation of oligonucleotide structure, as well as investigation of DNA hybridization, stability, and sequence‐dependent base nucleophilicity.
ISSN:1934-9270
1934-9289
DOI:10.1002/0471142700.nc0505s06