Protection of 5′‐Hydroxy Functions of Nucleosides

Protection of 5′‐Hydroxy Functions of Nucleosides

The 5‐hydroxy group is the primary hydroxy group of nucleosides. It is mandatory to protect 5‐hydroxyls in all methods of oligonucleotide synthesis that require nucleoside synthons. This unit discusses a wide variety of acid‐labile and base‐labile protecting groups, as well as enzymatic methods for...

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Veröffentlicht in:Current Protocols in Nucleic Acid Chemistry 2001-05, Vol.Chapter 2 (1), p.2.3.1-2.3.34
1. Verfasser: Seliger, H.
Format: Artikel
Sprache:eng
Schlagworte:
Acids
Acylation
Biochemistry - methods
Catalysis
Enzymes - metabolism
Fluorescent Dyes
Hydroxyl Radical - chemistry
Ligands
Nucleosides - chemistry
Phosphorylation
Trityl Compounds - chemistry
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Beschreibung
Zusammenfassung:The 5‐hydroxy group is the primary hydroxy group of nucleosides. It is mandatory to protect 5‐hydroxyls in all methods of oligonucleotide synthesis that require nucleoside synthons. This unit discusses a wide variety of acid‐labile and base‐labile protecting groups, as well as enzymatic methods for 5‐protection and deprotection.
ISSN:1934-9270
1934-9289
DOI:10.1002/0471142700.nc0203s00