Enzymatic Synthesis of M 1 G‐Deoxyribose
Adducts formed between electrophiles and nucleic acid bases are believed to play a key role in chemically induced mutations and cancer. M 1 G‐dR is an endogenous exocyclic DNA adduct formed by the reaction of the dicarbonyl compound malondialdehyde with a dG residue in DNA. It is an intermediate in...
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| Veröffentlicht in: | Current Protocols in Nucleic Acid Chemistry 2000-02 (1) |
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| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
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| Zusammenfassung: | Adducts formed between electrophiles and nucleic acid bases are believed to play a key role in chemically induced mutations and cancer. M
1
G‐dR is an endogenous exocyclic DNA adduct formed by the reaction of the dicarbonyl compound malondialdehyde with a dG residue in DNA. It is an intermediate in the synthesis of a class of modified oligodeoxyribonucleotides that are used to study the mutagenicity and repair of M
1
G. This unit presents methods for synthesizing M
1
G‐dR by enzymatic coupling. |
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| ISSN: | 1934-9270 1934-9289 |
| DOI: | 10.1002/0471142700.nc0102s00 |