Isolation of Neonatal Rat Cortical Astrocytes for Primary Cultures

Isolation of Neonatal Rat Cortical Astrocytes for Primary Cultures

There is increasing interest in the role of astrocytes as mediators of neurotoxicity. This unit describes a method for preparing astrocyte cultures of greater than 95% purity by enzymatic dissociation from neonatal rat brain. These preparations have both high yield and high viability.

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Bibliographische Detailangaben
Veröffentlicht in:Current Protocols in Toxicology 2001, Vol.4 (1), p.12.4.1-12.4.15
Hauptverfasser: Allen, Jeffrey W., Mutkus, Lysette A., Aschner, Michael
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Animals, Newborn
Astrocytes - cytology
Cell Culture Techniques
Cerebral Cortex - cytology
Culture Media
Rats
Rats, Sprague-Dawley
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Beschreibung
Zusammenfassung:There is increasing interest in the role of astrocytes as mediators of neurotoxicity. This unit describes a method for preparing astrocyte cultures of greater than 95% purity by enzymatic dissociation from neonatal rat brain. These preparations have both high yield and high viability.
ISSN:1934-9254
1934-9262
DOI:10.1002/0471140856.tx1204s04