Effects of Glucocorticoids on Subgingival Porphyromonas Gingivalis in Rats with Adriamycin-Induced Nephropathy

Objective:This paper aims to investigate the effect of glucocorticoid therapy on porphyromonas gingivalis(P.g)in subgingival plaque of rats with adriamycin-induced nephrotoxicity(ADR),and to realize the correlation between periodontitis nephrotic and glucocorticoid applications.Methods:60 Wistar rats were randomly divided into two groups and normal group,and nephropathy group,30 rats in each group.The rats in the nephropathy group were given 4 mg/kg adriamycin injection via tail vein to establish ADR model at the interval of 2 weeks.After the establishment of ADR model,the rats of the two groups were randomly selected 10 rats to be sacrificed and make the kidney pathological sections.Remaining 40 rats,20 rats in the normal group were randomly divided into control group and glucocorticoids-treated group,10 rats in each group.Nephropathy group were divided into ADR group and ADR+glucocorticoids-treated group,10 rats in each group.Glucocorticoids-treated group and ADR+glucocorticoids-treated group were given methylprednisolone 30 mg/(kg·d)for 10 weeks,control group and ADR group were given equal volume of normal saline.The subgingival plaque of rats was used to detect the detection rate and detection quantity of P.g in samples by using SYBR Green real-time quantitative PCR(qRT-PCR)technique.Results:The detection quantity of P.g in ADR+glucocorticoids-treated group was higher than that in other three groups(P<0.05),and the detection quantity of P.g in ADR group was higher than those in control group and glucocorticoids-treated group(P<0.05).The detection rates of P.g in control group and glucocorticoids-treated group were 40%and 50%respectively.The detection rate of P.g in ADR group and ADR+glucocorticoids-treated group was 90%,which was higher than that in control group and glucocorticoids-treated group(P<0.05).Conclusion:The level of P.g in rats was correlated with chronic kidney disease and the application of glucocorticoid,suggesting that the periodontal condition of ADR rats was affected by nephropathy and glucocorticoid therapy.