Effects of feeding E.adenophorum- containing feedstuffs on the cell cycle,apoptosis and mRNA expression of Bax,Bcl-2,Caspase- 3,8,9 of kidney in Saanen Goat

The experiment was conducted to explore the effects of feeding Saanen goat E.adenophorum-containing feedstuffs on apoptosis,cell cycle progression,and relative expression quantity of apoptosis genes of Bax,Bcl-2,caspase-3,8,9 in kidney through flow cytometry and qRT-PCR.Sixteen Saanen goats randomly divided into four groups were fed on control diet（non-E.adenophorum） and experimental diets mixed with 40%,60%,80% E.adenophorum leaves for 3 months respectively.As tested by flow cytometry,the results showed that the percentage of apoptosis in renal cell was increased and the normal renal cells obviously were decreased in experimental groups as compared to control.Renal cells in G0/G1 phase were much higher,and lower in S phase and proliferation index value in experimental groups which means E.adenophorum importantly arrest the cell cycle transition from G0/G1 to S phase which made renal cell can’t complete the cell cycle activity and increased cellular apoptosis in the kidney.qRT-PCR results suggested that E.ade