A SIMPLE, RAPID AND NEW METHOD FOR PREPARATION OF OLIGORIBONUCLEOTIDES

<正> Oligoribonucleotides have been prepared by using chemical synthesis and enzymatic synthesis or by means of partially hydrolyzing polyribonucleotides with alkaline and specific RNase. These methods have their own advantages and drawbacks. Although by the chemical method one can synthesize any oligoribonucleotides with the defined sequence, it takes a long period to prepare the chemically protected ribonucleosides and ribonucleotides. Partially hydrolyzing polyribonucleotides with alkaline and specific RNase is a simple and rapid way, but the vields are very low. because the resultant products are actually a mixture of oligor-