Effects of electronically stimulating Tianshu（ST 25） and Dachangshu（BL 25）, Quchi（LI 11） and Shangjuxu（ST 37） on the expressions of jejunum c-kit protein and c-kit m RNA in rats with functional diarrhea

OBJECTIVE： To investigate the effects of electronically stimulating Tianshu（ST 25） and Dachangshu（BL 25）, Quchi（LI 11） and Shangjuxu（ST 37） on the jejunum c-kit protein and c-kit m RNA in rats with functional diarrhea（FD）.METHODS： FD models were established through intragastric administration with folium sennae. Experimental rats were then divided into 4 groups：blank group, model group, electroacupuncture group Ⅰ [Tianshu（ST 25） and Dachangshu（BL 25）of both sides] and electroacupuncture group Ⅱ [Quchi（Li 11） and Shangjuxu（ST 37） ofboth sides], 10 in each. After treatment with electroacupuncture for 10 days, The expressions of jejunum c-kit protein and c-kit m RNA in each group were detected with Western blot and Real-Time quantitative real-time polymerase chain reaction（PCR）.RESULTS： The expressions of c-kit protein and c-kit m RNA in the model group increased significantly compared to those in the blank group（P 〈 0.01）;the expressions in electroacupuncture group Ⅰsignificantly decreased compared to those in the model group（P 〈 0.01）.CONCLUSION： Our findings suggest that electronically stimulating both Tianshu（ST 25） and Dachangshu（BL 25） significantly increased the expressions of jejunum c-kit protein and c-kit m RNA in FD rats, which means the treatment might have better therapeutic effects on FD.