Isoprenaline increases serum levels of monocyte chemoattractant protein-1 and tissue inhibitor of matrix metalloproteinases type I in Wistar rats

Background Treatment of rats with the beta-adrenergic agonist Isoprenaline（ISO） results in cardiac hypertrophy and myocardial fibrosis.In the present work,we aimed to study the in vivo effects of ISO on serum levels of monocyte chemoattractant protein- 1 and tissue inhibitor of matrix metalloproteinases type I in Wistar rats.Methods ISO（5 mg·kg-1） or Saline were injected subcutaneously into Wistar rats once a day for 3 or 7 consecutive days.Ventricular remodeling and cardiac function were evaluated by echocardiography.Sections of heart were stained with hematoxylin-eosin（HE） for histopathology or with Massons trichrome for collagen visualization.In addition,heart tissue immunohistochemistry for ɑ-SMA was also analyzed.The serum levels of tissue inhibitor of matrix metalloproteinases type I（TIMP-1） and monocyte chemoattractant protein-1（MCP-1） were determined by Luminex multiplex technology.Results ISO induced cardiac dysfunction in rats after 3 or 7 days of treatment.ISO caused significant increase of myocardial disorder and fibrosis withincreased ɑ-SMA expression.ISO treated aats showed a significant increase in the serum levels of TIMP- 1 and MCP-1.Conclusions Our study suggests that ISO induces profound cardiac remodeling accompanied with increase of serum TIMP-1and MCP-1.