H1-A, a compound isolated from Fusarium oxysporum inhibits hepatitis C virus （HCV） NS3 serine protease

The present study was aimed to isolate the active compounds from the fermentation products ofFusarium oxysporum, which had hepatitis C virus （HCV） NS3 protease inhibitory activity. A bioactive compound was isolated by reverse-phase silica-gel column chromatography, silica-gel column chromatography, semi-preparative reverse-phase High Performance Liquid Chromatography （HPLC）, and then its molecular structure was elucidated based on the spectrosopic analysis. As a result, the compound （HI-A, 1） Ergosta-5, 8 （14）, 22-trien-7-one, 3-hydroxy-（3fl, 22E） was isolated and identified. To the best of our knowledge, this was the first report on the isolation of Hi-A from microorganisms with the inhibitory activity ofNS3 protease.