Expressions of E-cadherin, pl20ctn, p-catenin and NF-KB in Ulcerative Colitis

This study was aimed to investigate the expressions of E-cadherin, p120 ctn, β-catenin and NF-κB in ulcerative colitis（UC） tissues and the implications of their expressions in the pathogenesis of UC. The expressions of E-cadherin, p120 ctn, β-catenin and NF-κB were detected by immunohistochemistry, and those of p120 ctn and NF-κB by Western blotting in 23 cases of UC and 17 cases of normal colonic tissues. The relationship between the expression of E-cadherin or NF-κB and that of p120 ctn was analyzed by Spearman rank correlation analysis. The results showed that in UC and normal colonic groups, the abnormal expression rate of E-cadherin, p120 ctn, β-catenin, and NF-κB was 52.2% vs. 0（P〈0.05）, 73.9% vs. 23.5%（P〈0.05）, 65.2% vs. 17.6%（P〈0.05） and 78.4% vs. 23.5%（P〈0.05）, respectively. p120 ctn expression was positively correlated with E-cadherin expression（r=0.404, P〈0.05）, but negatively with nuclear NF-κB expression（r= – 0.347, P〈0.05）. Western blotting showed that as compared with the normal controls, the p120 ctn protein level was significantly decreased（P〈0.05）, whereas the NF-κB protein level was increased（P〈0.05） in UC tissues. It was concluded that in the colonic tissues of UC patients, the expressions of E-cadherin, p120 ctn and β-catenin are decreased, suggesting the mucosal barrier is impaired in UC. Moreover, NF-κB is increased and activated in the UC tissues, resulting in the inflammation in UC. p120 ctn may influence the UC development through modulating intercellular adhesion and inflammatory response.