Complete genome sequence analysis of two Citrus tatter leaf virus(CTLV) isolates from China

In order to understand molecular characterization of Citrus tatter leaf virus(CTLV) isolated from China,full-length cDNAs of CTLV-MTH and CTLV-XHC from Citrus reticulata and Citrus sinensis were cloned and sequenced based on whole-genome amplification by RT-PCR.The complete nucleotide sequences of C...

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Veröffentlicht in:农业科学学报:英文版 2015 (5), p.984-987
1. Verfasser: SONG Zhen LI Zhong-an LIU Ke-hong ZHOU Chang-yong
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Sprache:eng
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Zusammenfassung:In order to understand molecular characterization of Citrus tatter leaf virus(CTLV) isolated from China,full-length cDNAs of CTLV-MTH and CTLV-XHC from Citrus reticulata and Citrus sinensis were cloned and sequenced based on whole-genome amplification by RT-PCR.The complete nucleotide sequences of CTLV-MTH and CTLV-XHC were determined to be 6497 nucleotides in length and shared 79.9-91.0%and 78.8-98.0%nucleotide sequence identity,respectively,with other Apple stem grooving virus(ASGV) or CTLV strains available in GenBank.Unexpectedly,CTLV-MTH showed the highest nucleotide sequence identity(91%) with an apple isolate of ASGV,followed by 86.5%with ASGV-HH and 85.7%with ASGV-CHN.Furthermore,CTLV-MTH and three ASGV strains were grouped to a separate cluster in the phylogenetic tree,suggesting it has a closer relationship to ASGV than to CTLV.Therefore,it can be concluded roughly that CTLV may be not a distinct strains of ASGV.We proposed that Citrus tatter leaf virus should be renamed Apple stem grooving virus.
ISSN:2095-3119
2352-3425