Research to Produce Ethanol from Seaweed Biomass Cladophora sp

Biomass seaweed Cladophora sp. （BSC） included Cladphora socialis, Cladophora prolifera, Cladophora crupila, which was hydrolysised in dilute sulfuric acid and enzymatic hydrolysis of cellulase. BSC was treated by dilute sulfuric acid at concentration of 0, 0.5%, 1%, 2%, 3%, 4%, 5%, 6% （v/v） （v： volume） in autoclave respectively at 121 ℃ at time 20, 40 and 60 minute, with the rate between seaweed and solution acid of 1/10（w/v） （w： weight）. Beside, BSC was pretreated in dilute sulfuric acid at 0.5%（v/v） at 121 ~C in 20 minute, after it was treat at concentrations 0, 0.2, 0.4, 0.6, 0.8, 1 ml/g with the enzymatic hydrolysis consisted of cellulase in control （50 ℃, pH 5.0, 24 h）. The results showed that, total sugar contents of two methods are the same, but sugar content was varied by treatment time of heat. Rates of acid 4%; 5%, 6% （v/v） and 0.8; 1 ml/g have high sugar content within the range of 42.38-48.5 1 mg/ml. 400 ml of hydrolysis solution was fermented by Saccharomyces cerevisiae at 30 ℃ for 72 h. After fe