Comparison of Extraction Methods for Genomic of Fall Webworm [ Hyphantria cunea (Drury)] Amplification Effect Sequences of CO I and of Different Primers CO II and Cytb DNA and on

[ Objective ] The paper was to compare different extraction methods for genomie DNA of fall webworm [ Hyphantria cunea (Drury) ] and study the am- plification effect of different primers on sequences of CO I and CO II and Cytb. [Method] With winter pupae of fall webworm as the test materials, genomi...

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Veröffentlicht in:Plant Diseases and Pests(植物病虫害研究:英文版) 2013, Vol.4 (4), p.36-38
1. Verfasser: Zhuang Mingjuan Chen Jingyun Zhan Guohui Cai Ping
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Sprache:eng
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Zusammenfassung:[ Objective ] The paper was to compare different extraction methods for genomie DNA of fall webworm [ Hyphantria cunea (Drury) ] and study the am- plification effect of different primers on sequences of CO I and CO II and Cytb. [Method] With winter pupae of fall webworm as the test materials, genomic DNA of fall webworm were extracted by five different methods including CTAB method, SDS method, improved SDS method, kit method and different processing time. Six groups of different primers were used to amplify sequences of CO I and CO II and Cytb. [ Result~ Full DNA could be extracted by CTAB method, SDS method and improved SDS method, and the effort of low concentration SDS method was the best. Six groups of primers could amplify the corresponding fragment, and two groups of primers had the best effort for amplification of CO I . [ Conclusion] The study provides better methods for DNA extraction.
ISSN:2152-3932