Improved biocompatibility of phosphorylcholine end-capped poly（butylene succinate）

In this work, the biocompatibility of a biomimetic, fully biodegradable ionomer phosphorylcholine （PC）-functionalized poly（butylene succinate） （PBS-PC） was investigated by means of hemolysis, platelet adhesion, protein adsorption and cytotox- icity experiments. The reference materials were poly（butylene succinate） （PBS） and chloroethylphosphoryl functionalized poly（butylene succinate） （PBS-Cl）. The hemolysis rates （HR） of the leaching solutions of PBS, PBS-Cl and PBS-PC were all lower than the safe value, and the rate of PBS-PC was reduced to 1.07%. Scanning electron microscopy （SEM） measurements showed that platelet adhesion and aggregation were significant on both PBS and PBS-Cl surface. In contrast, very few platelets were observed on PBS-PC surface. Bicinchoninic acid （BCA） measurements revealed that the adsorption amounts of bovine serum albumin （BSA） and bovine plasma fibrinogen （BPF） on PBS-PC surface were 52% and 72% reduction respectively compared with those on PBS surface. Moreover, non-cytotoxicity of both PBS-PC particles and its leaching solution was sug- gested by MTT assay using mouse L929 fibroblast cells. All the results demonstrated that the biocompatibility of PBS could be greatly improved by PC end-capping strategy. This PC functionalized polyester may have potential applications in biological environments as a novel carrier for controlled drug release and scaffold for tissue engineering.